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2 639 572

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IPC

C12Q1/68(2006-01-01)

A61L2/16(2006-01-01)

(21) (22)

Application

2016141505, 2016-10-21

(24)

Start date

2016-10-21

(22)

Actual filing date

2016-10-21

(45)

Published

2017-12-21

(72)

Inventor

Chernykh Oleg YurevichDzhailidi Georgij AnastasovichLysenko Aleksandr AnatolevichShevchenko Aleksandr AlekseevichKhakhov Latif Aslanbievich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Agrarnyj Universitet Imeni I.T. Trubilina"

METHOD FOR BIOMATERIAL PREPARATION TO CONTROL QUALITY OF SWINE HOUSE DISINFECTION FROM AFRICAN SWINE FEVER VIRUS Russian patent published in 2017 - IPC C12Q1/68 A61L2/16

Abstract RU 2639572 C1

FIELD: biotechnology.

SUBSTANCE: method provides sampling of manure, manure homogenization using sterile porcelain mortars and pestles, 10% suspension is prepared in sterile physiological saline or phosphate buffer. Centrifugation is carried out with suspension phase separation. The centrifuged suspension is allowed to stand and an aliquot of the supernatant is used to isolate the African swine fever virus DNA. DNA is detected by a polymerase chain reaction with a fluorescent detector in real time. If a fragment of the African swine fever virus genome is detected in at least one of the samples, all manure contained in the manure storage is re-disinfected.

EFFECT: invention allows to simplify the detection of African swine fever virus.

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RU 2 639 572 C1

Authors

Chernykh Oleg Yurevich

Dzhailidi Georgij Anastasovich

Lysenko Aleksandr Anatolevich

Shevchenko Aleksandr Alekseevich

Khakhov Latif Aslanbievich

Dates

2017-12-21—Published

2016-10-21—Filed