FIELD: medicine.
SUBSTANCE: immunohistochemical detection of markers of the outer mitochondrial membrane and mitochondrial complex IV is performed: histological samples-slices are prepared, a fluorescent signal amplifier is applied thereto, then a blocker protein is applied to the slices, providing reduction of background nonspecific staining, and then secondary fluorescent antibodies and a photoprotective reagent are applied with subsequent study using a luminescent microscope.
EFFECT: implementation of the invention allows to increase the reliability of the central rod myopathy diagnosis by expanding the technical capabilities of study of the skeletal striated muscles biopsy specimens by the immunohistochemical method on paraffin biopsy preparations.
2 dwg,1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DIAGNOSTICS OF CONGENITAL NEMALINE MYOPATHY | 2016 |
|
RU2630982C1 |
| METHOD OF DIAGNOSTICS OF AUTOIMMUNE VIOLENT DERMATOSES | 2008 |
|
RU2352947C1 |
| METHOD OF DIFFERENTIAL DIAGNOSIS OF RED LICHEN PLANUS AND LYCEOPLACY OF THE ORAL MUCOSA | 2017 |
|
RU2651050C1 |
| METHOD FOR PREDICTION OF MYOMATOUS ASSEMBLY EXPULSION FOLLOWING UTERINE ARTERY EMBOLIZATION IN A PATIENT WITH UTERINE MYOMA | 2019 |
|
RU2701210C1 |
| ESTIMATE OF TISSUE ADAPTATION LEVEL TO ORGAN AND SYSTEM INCOMPETENCE BY EVALUATING COMPENSATORY PROLIFERATION OF MITOCHONDRIA IN RELATED TISSUE CELLS | 2007 |
|
RU2357247C1 |
| METHOD FOR DIFFERENTIAL DIAGNOSTICS OF ACTINIC KERATOSIS AND SQUAMOUS CELL CARCINOMA OF SKIN | 2019 |
|
RU2703464C1 |
| METHOD OF IMMUNOHISTOCHEMICAL ANTIGENS DETECTION IN PREPARATIONS OF PRODUCTIVE AND NON-PRODUCTIVE ANIMALS ORGANS OF LONG-TERM STORAGE IN FIXTURES | 2016 |
|
RU2627448C1 |
| CELL PRODUCT OF INSULIN-PRODUCING MAMMALIAN CELLS AND USE THEREOF FOR DIABETES MELLITUS THERAPY | 2017 |
|
RU2663118C1 |
| EARLY DIAGNOSTIC TECHNIQUE FOR DEVELOPING PELVIC PROLAPSE IN FEMALES OF REPRODUCTIVE AGE WITH NO CLINICAL SIGNS | 2012 |
|
RU2498308C1 |
| ANTIBODY RAISED AGAINST HUMAN MITOCHONDRIAL ISOZYME ADENYLATE KINASE, DIAGNOSTIC COMPOSITION AND DIAGNOSTIC SET FOR DIAGNOSIS OF HEART DISEASE | 2000 |
|
RU2262939C2 |
