METHOD FOR PRODUCTION OF VACCINE AGAINST BRUCELLOSIS OF SMALL CATTLE Russian patent published in 2018 - IPC C12N1/20 A61K39/10 C12R1/01 

FIELD: biotechnology.

SUBSTANCE: method for production of vaccine against the brucellosis of small cattle provides seeding and growing strain of Brucella melitensis Rev-1 on a nutritional medium, containing pancreatic digest of casein of a high decomposition degree, yeast extract, sodium chloride, glucose, glycerol, and agar-agar in the specified component ratio within 72 hours at 37°C. The grown vaccine culture is washed from the surface of the agar by a drying medium and brought to a concentration of 80-140 billion m.c. per 1 ml. It is checked for purity and dissociation, packed in ampoules and subjected to freeze-drying.

EFFECT: increase in the yield of Brucella melitensis Rev-1 strain in the S-form.

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