FIELD: biotechnologies.
SUBSTANCE: invention relates to the method to produce a brucellosis antigen for the rose bengal test (RBT). The method to produce the brucellosis antigen includes growing of the strain B. abortus 104M on the solid nutrient medium, washing of the culture with 0.5% phenolized solution, inactivation of biomass in a water bath at the temperature of 88±1°C for 30 minutes, performance of inspection for sterility, whirling of the bacterial suspension at 6-7 K rpm for 40 minutes, slurrying of the brucelli residue in 0.7% water solution of rose bengal at the ratio of 1:1 and dyeing of microbial cells at 4°C, re-slurrying of the microbial suspension in the buffer dissolvent and performance of standardisation.
EFFECT: method makes it possible to produce an antigen of higher activity and may be used in production of preparations for brucellosis diagnostics.
3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| WAY OF RECEPTION OF BRUCELLOUS ANTIGEN FROM STRAIN BRUCELLA ABORTUS 19 FOR MANUFACTURING OF UNIFORM BRUCELLOUS ANTIGEN FOR AA (AGGLUTINATION ASSAY), CFR (COMPLEMENT FIXATION REACTION) AND LCFR (LONG-TERM COMPLEMENT FIXATION REACTION), BRUCELLOUS ANTIGEN FOR ROSE BENGAL ASSAY (RBA) AND BRUCELLOUS ANTIGEN FOR RING TEST (RT) WITH MILK, WAY OF MANUFACTURING OF BRUCELLOUS DIAGNOSTIC SERUM AND DIAGNOSTIC SETS | 2008 |
|
RU2361610C1 |
| METHOD FOR PREPARING CONCENTRATED BRUCELLA CELL CULTURE FROM Brucella abortus 19 STRAIN TO PREPARE BRUCELLOUS ANTIGENS, BRUCELLOUS ANTIGENS (THREE VERSIONS), METHOD OF MAKING BRUCELLOSIS DIAGNOSTIC SERUM AND TEST SYSTEM FOR DIAGNOSING BRUCELLOSIS IN ANIMALS (THREE VERSIONS) | 2014 |
|
RU2593712C2 |
| METHOD FOR OBTAINING ANTIGENS FOR THE PURPOSES OF BRUCELLOSIS SEROLOGIC DIAGNOSIS | 0 |
|
SU1713592A1 |
| METHOD OF ANTIGEN PREPARING FOR BRUCELLOSIS DIAGNOSIS | 1997 |
|
RU2133470C1 |
| METHOD FOR MAKING BRUCELLOSIS DIAGNOSTIC SERUM | 2013 |
|
RU2549434C2 |
| STRAIN OF BACTERIUM BRUCELLA ABORTUS USED FOR PREPARING DIAGNOSTIC AND PROPHYLACTIC BIOPREPARATIONS AGAINST BRUCELLOSIS IN ANIMALS | 1999 |
|
RU2149183C1 |
| METHOD FOR PRODUCING BRUCELLOUS L-ANTIGEN | 2011 |
|
RU2486916C2 |
| METHOD FOR OBTAINING THE R-BRUCELLOSIS SERUM ON RABBITS | 2017 |
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RU2659948C1 |
| METHOD OF OBTAINING A BRUCELLAROTIC ANTIGEN FOR CELL TESTS IN VITRO | 2019 |
|
RU2708561C1 |
| METHOD FOR PRODUCING R-BRUCELLAR ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHGT) | 2008 |
|
RU2411041C2 |
