FIELD: biotechnology.
SUBSTANCE: invention is a method for producing an allergen for diagnosing brucellosis in farm animals, including: accumulation of the bacterial mass of a Brucella culture, thermal inactivation and separation of the bacterial mass from the culture medium, alkaline hydrolysis of the bacterial mass, separation of the hydrolysate from the hydrolysed bacterial mass, combining the culture medium filtrate with the hydrolysate, adjusting the protein concentration in the target product to the working value.
EFFECT: invention makes it possible to obtain an allergen for an allergic intradermal test in farm animals, to expand the arsenal of methods for obtaining allergens for diagnosing brucellosis in farm animals.
1 cl, 3 tbl, 28 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING A BRUCELLAROTIC ANTIGEN FOR CELL TESTS IN VITRO | 2019 |
|
RU2708561C1 |
| METHOD OF PRODUCING A BRUCELLOUS ERYTHROCYTE ANTIGEN FOR THE INDIRECT HEMAGGLUTINATION REACTION (IHAR) | 2019 |
|
RU2714305C1 |
| METHOD FOR DETECTION OF GERM CARRIER IN CATTLE BRUCELLOSIS | 1989 |
|
RU2008918C1 |
| POLYVALENT CORPUSCULAR ANTIGEN FOR PRODUCTION OF MEDICAL AND PREVENTIVE BIOLOGICAL PRODUCTS FOR BRUCELLOSIS | 2006 |
|
RU2330681C1 |
| 0 |
|
SU1567984A1 | |
| METHOD FOR PROPHYLACTICS OF CATTLE BRUCELLOSIS | 2008 |
|
RU2378011C1 |
| METHOD FOR OBTAINING OF ANTIGEN FOR DETERMINATION OF ANTIBRUCELLAR IMMUNITY | 2016 |
|
RU2627897C1 |
| METHOD FOR PREPARING CONCENTRATED BRUCELLA CELL CULTURE FROM Brucella abortus 19 STRAIN TO PREPARE BRUCELLOUS ANTIGENS, BRUCELLOUS ANTIGENS (THREE VERSIONS), METHOD OF MAKING BRUCELLOSIS DIAGNOSTIC SERUM AND TEST SYSTEM FOR DIAGNOSING BRUCELLOSIS IN ANIMALS (THREE VERSIONS) | 2014 |
|
RU2593712C2 |
| METHOD TO PRODUCE BRUCELLOSIS ANTIGEN FOR ROSE BENGAL TEST (RBT) | 2011 |
|
RU2488119C2 |
| METHOD FOR PRODUCING BRUCELLOUS L-ANTIGEN | 2011 |
|
RU2486916C2 |
