HYDROPHILIC POLYMER PARTICLES AND METHODS FOR THEIR OBTAINING Russian patent published in 2018 - IPC C08F2/16 C08F220/54 C08F8/12 C08F222/38 C12Q1/68 C08F2/20 C08F2/26 C08F230/08 C08F257/02 C08F220/58 C08F220/60 C08F8/34 C08F130/08 C07F7/18 

FIELD: chemistry.

SUBSTANCE: initially, seed particles comprising a hydrophobic polymer are introduced into an aqueous suspension. Then, the seed particles are promoted in the aqueous suspension to form a dispersed phase. Thereafter, a hydrophilic monomer comprising an acrylamide monomer having a hydrophobic protecting group in a dispersed phase, with a diacrylamide crosslinker having a hydrophobic protecting group, is polymerized. A polymer particle is obtained which is a hydrogel particle comprising a plurality of hydrophobic protecting groups. At least a portion of the hydrophobic protecting groups is removed from the polymer particle and a hydrophilic particle is obtained. Then, an oligonucleotide is bound to the hydrophilic particle. In one embodiment of the process, a plurality of hydrophobic protecting groups is cleaved from the hydrophilic polymer obtained in the polymerization step and the hydrophobic polymer is extracted from the polymer particle to obtain a hydrogel particle.

EFFECT: invention allows to obtain particles that can effectively bind to polynucleotides.

73 cl, 2 dwg, 1 tbl, 25 ex