MODIFIED RHODAMINE DYE AND ITS USE IN BIOLOGICAL ANALYSIS Russian patent published in 2023 - IPC C07D491/153 C07D491/16 C07D491/20 C07H21/02 C07H21/04 C12N15/113 C12N15/65 C12Q1/6844 C09B11/24 G01N33/58 

FIELD: organic chemistry.

SUBSTANCE: group of inventions includes a compound of formula (I), where R¹, R², R³, R⁴, R⁶, R⁸, R¹², R^d and R^e represent hydrogen; R¹¹, R¹³ and R¹⁴ are -R^b; R⁵ and R¹⁰ are -C(O)R¹⁵, where R¹⁵ represents -CX₃; R^b is selected from -X and -C(O)OH; X represents halogen; and the dotted line indicates that there may be a double bond at the indicated position. The group of inventions also includes a reagent for labeling an oligonucleotide of the formula LM-L-PEP (XX), an oligonucleotide containing a tag fragment obtained by interaction of the oligonucleotide attached to a solid carrier with a reagent having the structure of the formula (XX), a method of simultaneous amplification and analysis of multiple genetic loci and a set containing oligonucleotide primers for co-amplification of a set of genetic loci. Where in formula (XX) PEP represents the phosphate ester precursor group of formula (P.1), LM represents the N-protected NH-rhodamine moiety of the molecule of formula (I), L represents the linker of formula –NH-(CH₂)₄-CH-(CH₂-O-DMT)-CH₂-O-.

(I) (Р.1)

EFFECT: fluorescent dyes of the rhodamine series of formula (I) used for labeling synthetic oligonucleotides.

25 cl, 13 dwg, 3 tbl, 3 ex