THREE-DIMENSIONAL POLYMER STRUCTURES ON FLOW CELLS Russian patent published in 2025 - IPC B01J19/00 C12Q1/6806 C08F2/48 

FIELD: biotechnology.

SUBSTANCE: invention relates to sequencing methods, namely to a method for sequencing on three-dimensional polymer structures in a cuvette and a method for producing such a cuvette. Method of making three-dimensional polymer structures on flow cells includes several steps. First, the polymer precursor solution is loaded into a flow cell suitable for use in sequencing. Polymer precursor solution contains a monomer, a cross-linking agent and a photoinitiator. Polymer precursor solution includes polyethylene glycol (PEG)-thiol, PEG-acrylate, acrylamide, N, N'-bis(acryloyl)cystamine (BACy), PEG, polypropylene oxide (PPO), polyacrylic acid, poly(hydroxyethyl methacrylate) (PHEMA), poly(methyl methacrylate) (PMMA), poly(N-isopropylacrylamide) (PNIPAAm), poly(lactic acid) (PLA), copolymer of lactic acid and glycolic acid (PLGA), polycaprolactone (PCL), poly(vinyl sulphonic acid) (PVSA), poly(L-aspartic acid), poly(L-glutamic acid), polylysine, agar, agarose, alginate, heparin, alginate sulphate, dextran sulphate, hyaluronan, pectin, carrageenan, gelatine, chitosan, cellulose, collagen, bisacrylamide, diacrylate, diallylamine, triallylamine, divinylsulphone, diethylene glycol diallyl ether, ethylene glycol diacrylate, polymethylene glycol diacrylate, polyethylene glycol diacrylate, trimethylopropane trimethacrylate, ethoxylated trimethylol triacrylate, ethoxylated pentaerythritol tetracrylate or combinations thereof. Flow cell has at least one channel for receiving the polymer precursor solution, and at least one channel has an upper inner surface and a lower inner surface. Polymer precursor solution is then exposed to light with a wavelength sufficient to activate the photoinitiator through a photomask with a given pattern, wherein upon activation of the photoinitiator, at least a portion of the polymer precursor solution under the holes in the photomask is polymerised and three-dimensional polymer structures are formed, which extend from the upper inner surface to the lower inner surface of at least one channel.

EFFECT: proposed approach enables to automate the sequencing process and reduce data loss associated with enrichment of the sequencing library.

19 cl, 46 dwg, 2 tbl, 6 ex