FIELD: biotechnology.
SUBSTANCE: method for conjugation of a substrate with a biomolecular complex and a method for conjugation of a polymer particle with a polynucleotide complex are proposed. A method for conjugation of a substrate with a biomolecular complex involves exchanging a counterion-bound counterion with a lipophilic counterion to produce a biomolecular complex, dispersing the biomolecular complex in a non-reactive polar non-aqueous solvent, and binding the biomolecular complex to the substrate in the presence of a non-reactive polar non-aqueous solvent. A method for conjugation of a polymer particle with a polynucleotide complex comprises exchanging a cationic counterion bound to a polynucleotide with a lipophilic cation counterion to produce a polynucleotide complex, dispersing the polynucleotide complex in a non-reactive polar non-aqueous solvent, and binding the polynucleotide to the polymer particle via nucleophilic or electrophilic substitution.
EFFECT: improved conjugation method.
17 cl, 6 dwg, 4 ex
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