ANTIBODIES AGAINST NERVE GROWTH FACTOR AND METHODS FOR THEIR PRODUCTION AND APPLICATION Russian patent published in 2018 - IPC C07K16/22 C07K16/46 A61K39/395 A61P29/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method for production of antibodies suitable for use in a dog that specifically bind to a canine nerve growth factor (NGF) and neutralise the ability of canine NGF to bind to the canine NGF receptor p75 or TrkA, and to the corresponding antibodies. To implement this method, a donor antibody is obtained from a species other than a dog, the donor antibody shall have binding specificity for the target antigen present in dogs. Then, each amino acid residue in the sequence of the framework regions of the donor antibody is compared with each amino acid residue present at the corresponding position in the sequence of the framework regions of the canine antibody pool. Then, the identified amino acid residues in the donor antibody are replaced with amino acid residues present at the corresponding position in the canine antibody pool. The resulting antibody does not contain any amino acid that will be foreign in the corresponding position in the canine antibody in any position within the framework regions. This invention also discloses a pharmaceutical composition and kit containing the said antibodies for treatment of pain in dogs, in particular pain associated with immuno-mediated polyarthritis, osteoarthritis or rheumatoid arthritis.

EFFECT: invention avoids reduction or loss of antigen-binding activity in the production of canine antibodies.

23 cl, 19 dwg, 8 tbl, 14 ex