ANTIBODIES AGAINST NERVE GROWTH FACTOR AND METHODS FOR THEIR PRODUCTION AND APPLICATION Russian patent published in 2017 - IPC C07K16/22 C07K16/46 A61K39/395 A61P29/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method for production of antibodies suitable for use in horses that are specifically associated with the horse nerve growth factor (NGF) and neutralise the ability of the horse NGF to contact the horse NGF p75 or TrkA receptor and the corresponding antibodies. To implement this method, a donor antibody is obtained from a species other than a horse, the donor antibody having a binding specificity for the target antigen present in horses. Then, each amino acid residue in the sequence of the framework regions of the donor antibody is compared to each amino acid residue present at the corresponding position in the sequence of the framework regions of horse antibodies pool. Then, the identified amino acid residues in the donor antibody are replaced with the amino acid residues present at the corresponding position in the pool of horse antibodies. The resulting antibody does not contain any amino acid foreign at the corresponding position in horses, at any position within the framework regions. This invention also discloses a pharmaceutical composition and a kit containing the said antibodies for the treatment of pain or an NGF-induced tumour in horses.

EFFECT: decrease or loss of antigen-binding activity is avoided.

12 cl, 11 dwg, 8 tbl, 10 ex