SINGLE-CHAIN ANTIBODIES AND OTHER HETEROMULTIMERS Russian patent published in 2018 - IPC C07K16/46 

FIELD: biotechnology.

SUBSTANCE: single-chain antibody for treatment or diagnosis comprising a single polypeptide containing the following domains located relative to each other from the N-terminus to the C-terminus is provided as follows: VL₁-CL₁-CLH linker1-VH₁-CH1₁-sharnir1-CH2₁-CH3₁-HD linker-VL₂-CL₂-CLH linker2-VH₂-CH1₂-sharnir2-CH2₂-CH3₂, where each of the CLH linker1, CLH linker2 and HD linker contains an amino acid sequence cleaved by the endopeptidase at the N- and C-termini. Also, a polynucleotide encoding this single-chain antibody, a vector comprising such a polynucleotide, a host cell comprising this vector, a method for production of this single-chain antibody by the said host cell are presented.

EFFECT: invention extends the arsenal for antibodies production.

112 cl, 6 dwg, 3 ex