METHOD OF MANUFACTURE OF FORMOLVACCINE OF HYDROOXYALUMINUM POLYSTAMMAL AGAINST STREPTOCOCCAL DISEASES OF LARGE CATTLE Russian patent published in 2018 - IPC A61K39/09 C12N1/20 A61K47/02 

FIELD: veterinary medicine.

SUBSTANCE: method of manufacturing a vaccine against streptococcal bovine diseases includes cultivation in a nutrient medium of microorganisms Str. agalactiae 346 (serogroup B), Str. zooepidemicus 2082 (serogroup C), Ent. Faecalis (serogroup D), inactivation of microorganisms and addition of an aluminum hydroxide adjuvant. At the same time, microorganisms are taken in the vaccine in equal volumes at initial concentrations of 17.5-25.5 billion m.t./cm³, cultivation of cultures of strains of streptococci is carried out at oxidation-reduction potential of a culture liquid -200÷-150 mV, until the end of the cultivation process, pO₂ in the culture liquid at a level of 15-25% of the saturation with air oxygen, the pH of the culture liquid is adjusted at the level of 6.8-7.2, and the fractional glucose supply is carried out in doses up to a concentration of 0.05-0.2% when the streptococcal growth is limited by glucose, Inactivation of streptococcal cultures is carried out with formaldehyde at a temperature of 43-47°C for 3-5 days, the final concentration of formaldehyde in culture being 0.03-0.07%.

EFFECT: decrease in the number of diseased calves and abortions, metritis and mastitis in adult animals.

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