Show metadata Hide metadata

(19)

(11)

2 404 246

(13)

(51)

IPC

C12N15/12(2006-01-01)

C12N15/70(2006-01-01)

(21) (22)

Application

2009123238/10, 2009-06-18

(24)

Start date

2009-06-18

(22)

Actual filing date

2009-06-18

(45)

Published

2010-11-20

(72)

Inventor

Ljukmanova Ekaterina NazymovnaShulepko Mikhail Anatol'EvichShenkarev Zakhar OlegovichDolgikh Dmitrij AleksandrovichTikhonov Roman VladimirovichKasheverov Igor' Evgen'EvichTsetlin Viktor IonovichKirpichnikov Mikhail Petrovich

(73)

Holder

Uchrezhdenie Rossijskoj Akademii Nauk Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Ran

RECOMBINANT PLASMID DNA pET22b(+)/"вд"-LINKS 1, CODING PROTEIN WITH LINKS 1 PROPERTIES, AND STRAIN OF Escherichia coli BL21 (DE3)/pET22b(+)/"вд"-LINKS 1 - PRODUCENT OF PROTEIN WITH PROPERTIES OF HUMAM LINKS 1 Russian patent published in 2010 - IPC C12N15/12 C12N15/70

Abstract RU 2404246 C1

FIELD: medicine.

SUBSTANCE: there is constructed recombinant plasmid protein-coding DNA pET22b(+)/"вд"-Links 1, which contains Ndel/BamHI -fragment of DNA of pET22b(+) plasmid and artificial DNA sequence, coding Ndel/BamHI fragment, which includes synthetic gene of human water-soluble domain Links 1, containing unique sites of recognition by restriction endonucleases, which have the following coordinates: Xhol - 158, Hindlll - 173, EcoRI - 192, BamHI - 198, Ndel - 430, Bglll - 534, Mlul - 1256, Pstl - 4495. Strain - protein-producent is obtained by transformation of competent cells of Escherichia coli BL21 (DE3) with recombinant plasmid pET22b(+)/"вд"-Links 1.

EFFECT: invention allows to obtain protein with structure and properties identical to structure and properties of natural water-soluble domain of human protein Links 1 in sufficient amount, measured in milligrams.

2 cl, 1 tbl, 4 dwg, 4 ex

Similar patents RU2404246C1

Title	Year	Author	Number
RECOMBINANT PLASMID DNA pET22b(+)/SLURP-1 CODING SLURP-1 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET22b(+)/SLURP-L-PRODUCENT OF HUMAN SLURP-1 PROTEIN	2010	Shulepko Mikhail Anatol'Evich Ljukmanova Ekaterina Nazymovna Lobas Anna Aleksandrovna Dolgikh Dmitrij Aleksandrovich Kirpichnikov Mikhail Petrovich	RU2453602C2
RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli	2013	Petrovskaja Lada Evgen'Evna Shingarova Ljudmila Nikolaevna Gapizov Sultan Shakhbanovich Krjukova Elena Aleksandrovna Boldyreva Elena Filippovna Jakimov Sergej Aleksandrovich Dolgikh Dmitrij Aleksandrovich Kirpichnikov Mikhail Petrovich	RU2527171C1
RECOMBINANT PLASMID DNA PES4-4 ENCODING HUMAN POLYPEPTIDE INTERFERON ALPHA-2B AND STRAIN ESCHERICHIA COLI BREES4 AS PRODUCER OF RECOMBINANT HUMAN INTERFERON ALPHA-2B	2003	Bairamashvili D.I. Vorob'Ev I.I. Gabibov A.G. Demin A.V. Miroshnikov A.I. Mart'Janov V.A. Ponomarenko N.A. Shuster A.M.	RU2258081C1
RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE	2015	Petrovskaya Lada Evgenevna Shingarova Lyudmila Nikolaevna Kryukova Elena Aleksandrovna Boldyreva Elena Filippovna Zlobinov Aleksandr Vladimirovich Dolgikh Dmitrij Aleksandrovich Kirpichnikov Mikhail Petrovich	RU2606014C1
RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b	2004	Bairamashvili D.I. Vorob'Ev I.I. Gabibov A.G. Demin A.V. Miroshnikov A.I. Mart'Janov V.A. Ponomarenko N.A. Shuster A.M.	RU2261913C1
RECOMBINANT PLASMID DNA pET22b(+)/slurp-2 CODING SLURP-2 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3) pET22b(+)/slurp-2 PRODUCER OF HUMAN SLURP-2 PROTEIN	2015	Ljukmanova Ekaterina Nazymovna Shulepko Mikhail Anatolevich Shenkarev Zakhar Olegovich Dolgikh Dmitrij Aleksandrovich Kirpichnikov Mikhail Petrovich	RU2583307C2
RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN	2019	Myagkikh Igor Valentinovich Stepanenko Vasilij Nikolaevich Makarov Dmitrij Aleksandrovich Sokolova Irina Vladimirovna Vorobeva Tatyana Vladimirovna Levandovskaya Kristina Georgievna Sveshnikova Elena Vasilevna Zinchenko Aleksej Alekseevich Pavlenko Daniil Mikhajlovich Nokel Elena Adamovna Melikhova Tatyana Dmitrievna Dolgikh Dmitrij Aleksandrovich Gasparyan Marine Eduardovna Miroshnikov Anatolij Ivanovich Korolev Ivan Romanovich Kalinin Danil Sergeevich Puchkov Ilya Aleksandrovich Novozhilov Nikolaj Mikhajlovich Kirpichnikov Mikhail Petrovich	RU2716975C1
WAY OF RECEPTION FUNCTIONALLY ACTIVE RECOMBINANT PROTEIN OF ULCER (LF) LETHAL FACTOR, RECOMBINANT PLASMIDE DNA pETHIS-LF CODING ACTIVE PROTEIN LF; H STRAIN ESCHERICHIA COLI BL-HISLF PRODUCING ACTIVE PROTEIN OF MALIGNANT ANTHRAX LETHAL FACTOR	2007	Kolesnikov Aleksandr Vladimirovich Zakharova Marija Jur'Evna Kozyr' Arina Vladimirovna Shemjakin Igor' Georgievich	RU2361921C1
ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN	2012	Ramazanov Jurij Akhmetovich Beklemishev Anatolij Borisovich	RU2512527C2
RECOMBINANT PLASMID DNA PES3-7 ENCODING PEPTIDE WITH SEQUENCE OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND STRAIN ESCHERICHIA COLI BL21(DE3)/PES3-7 AS PRODUCER OF HUMAN RECOMBINANT G-CSF	2003	Gabibov A.G. Ponomarenko N.A. Vorob'Ev I.I. Demin A.V. Mart'Janov V.A. Shuster A.M. Bairamashvili D.I. Miroshnikov A.I.	RU2260049C2

RU 2 404 246 C1

Authors

Ljukmanova Ekaterina Nazymovna

Shulepko Mikhail Anatol'Evich

Shenkarev Zakhar Olegovich

Dolgikh Dmitrij Aleksandrovich

Tikhonov Roman Vladimirovich

Kasheverov Igor' Evgen'Evich

Tsetlin Viktor Ionovich

Kirpichnikov Mikhail Petrovich

Dates

2010-11-20—Published

2009-06-18—Filed