FUSION PROTEINS AND METHODS OF TREATMENT, PREVENTION OR ALLEVIATION OF PAIN Russian patent published in 2018 - IPC C12N15/62 A61K38/00 A61K38/16 C07K19/00 C07K14/575 

FIELD: biochemistry; biotechnology.

SUBSTANCE: invention relates to biochemistry and biotechnology, in particular to non-cytotoxic analgesic single-chain polypeptide fusion protein. Present fusion protein comprises non-cytotoxic protease, first spacer, protease cleavage site, guiding fragment of galanin, second spacer, and translocation domain. This translocation domain transfers protease from endosome through endosomal membrane to cytosol of nociceptive sensory afferent. This protein differs in that first spacer is between non-cytotoxic protease and protease cleavage site, it is flexible and consists of 4-25 amino acid residues. Invention also relates to obtaining method of fusion protein and preparation of double-stranded non-cytotoxic analgesic polypeptide by cleavage of the single-stranded protein fusion protein. Present invention also relates to methods of treating, preventing or alleviating pain, including insertion of this fusion protein or a double-stranded polypeptide.

EFFECT: present invention provides to obtain polypeptides for treatment, prevention or alleviation of pain.

22 cl, 9 dwg, 10 ex, 5 tbl