HYBRID NEUROTOXINS Russian patent published in 2022 - IPC C07K14/33 C07K14/28 C07K19/00 C12N15/09 C12N15/63 

FIELD: biotechnology; biochemistry.

SUBSTANCE: invention relates to the field of biotechnology and biochemistry, specifically to hybrid polypeptide for binding to ganglioside. Hybrid polypeptide contains a light chain of clostridium neurotoxin (L), HN domain of clostridium neurotoxin, HCN domain of clostridium neurotoxin, HCC domain of clostridium neurotoxin with amino acid sequences at least 95% identical to sequences with amino acid residues, respectively, 1-448 SEQ ID NO: 1, 449-872 SEQ ID NO: 1, 873-1094 SEQ ID NO: 1, 1095-1296 SEQ ID NO: 1; and a selective ganglioside-binding fragment capable of binding to GM1, with an amino acid sequence at least 95% identical to a sequence with amino acid residues 22-124 SEQ ID NO: 9. The invention also relates to a nucleotide sequence encoding the specified hybrid polypeptide, an expression vector containing the specified sequence, and a cell for expression of the specified hybrid polypeptide, containing the specified vector.

EFFECT: invention allows for increase in selectivity of affinity of ganglioside binding.

11 cl, 6 dwg, 1 tbl, 2 ex