METHODS FOR DETECTION OF ANTI-CYTOMEGALOVIRUS NEUTRALIZING ANTIBODIES Russian patent published in 2018 - IPC C12Q1/70 

FIELD: chemistry.

SUBSTANCE: proposed is a neutralization assay method to evaluate candidate vaccines for their ability to interfere with HCMV (human cytomegalovirus). Method includes the following steps: (i) mixing heat inactivated serum from a subject that has been immunized with an HCMV candidate vaccine with an HCMV comprising a fluorescent moiety to form a mixture; (ii) adding 2.5% rabbit complement to the mixture from step (i); (iii) contacting a host cell that is susceptible to infection by HCMV, wherein said host cell is a retinal pigmented epithelial cell of ARPE-19, under conditions that allow infection with the mixture of heat inactivated serum in the presence of rabbit complement from step (ii); (iv) assessing a fluorescence level of the host cell that has been contacted with the mixture by flow cytometry; and (v) determining a level of infection of the host cell based on the assessed fluorescence level.

EFFECT: invention allows to evaluate potential candidate vaccines for neutralizing antibody induction.

20 cl, 5 dwg, 8 tbl, 4 ex