METHOD FOR OBTAINING ENTEROBACTERIUM ESCHERICHIA COLI OR SALMONELLA BIOMASS IN PRODUCTION BIOREACTORS Russian patent published in 2021 - IPC C12N1/20 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular, to the cultivation of microorganisms in production conditions. A method for obtaining the biomass of Enterobacteria Escherichia coli or Salmonella is proposed. The method includes the preparation of a nutrient medium based on a 0.9% solution of sodium chloride and a nutrient medium concentrate containing dry glucose, the main Hottinger digest, sodium phosphoric acid 12-water, magnesium sulfuric acid 7-water at specified amounts, inoculation of the nutrient medium with Escherichia coli or Salmonella bacteria and cultivating them under oxygen restriction at certain parameters with a sequential supply of the nutrient medium concentrate. The sequential feed of the nutrient medium concentrate is carried out in 3 stages. The first feed is carried out 2 hours after the inocultion in the amount of 2*V₀/90 l/bioreactor, the second feed - 3 hours after the start of growth in the amount of 4*V₀/90 l/bioreactor, and the third-starting from 3.5 hours of feeding 1,7*V₀/90 ml/sec in a constant mode, wherein V₀ is the initial volume of saline solution in the bioreactor, varying within the range of 90-300 l.

EFFECT: invention makes it possible to increase Escherichia coli and Salmonella bacteria biomass yield.

3 cl, 1 tbl, 5 ex, 2 dwg