FIELD: biotechnology; medicine.
SUBSTANCE: invention relates to biotechnology and immunology. Method for the preparation of AmpT protein for simulating anti-cholera immunity in experimental animals is proposed. Method involves growing an avirulent strain of V. cholerae eltor 18950, washing the cells, followed by precipitation, destruction in an ultrasound disintegrant, treatment with DNA-ase and RNA-ase, and centrifugation. Supernatant is ultracentrifuged, the pellet of membrane proteins is dissolved in 1 % Triton X-100, ultracentrifuged. OmpT protein is isolated from the supernatant liquid previously pre-dialyzed against 20 mM Tris-HCl (pH 7.6), applying to the column with DE-52 cellulose. Proteins sorbed on the cellulose are eluted with a gradient of NaCl.
EFFECT: method provides an effective preparation for modeling immunity in experimental animals.
4 cl, 1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CHOLERA PROPHYLAXIS METHOD USING VESICLES TO MODEL CHOLERA IMMUNITY IN RESEARCH ANIMALS | 2022 |
|
RU2792160C1 |
| AVIRULENT STRAIN OF BACTERIA Vibrio cholerae BIOVAR eltor, SEROVAR Ogava (ctxA-, tcpA-, toxR-, zot-), USED IN IMMUNOLOGICAL AND GENETIC INVESTIGATIONS AND EDUCATORY PROCESS | 2004 |
|
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| MODE OF ISOLATING PROTEINS OF TOXIN-COREGULATED ADHESION PILI AND OmpU OF COMMA BACILLUS OF CLASSIC BIOVAR | 2006 |
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RU2324740C2 |
| CULTIVATED HYBRID CELL STRAIN OF Mus musculus L ANIMALS - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO O-ANTIGENE OF CHOLERA VIBRIO SEROGROUP O1 | 2010 |
|
RU2425874C1 |
| AVIRULENT STRAIN OF Vibrio cholerae KM 263 BACTERIA OF SEROVAR INABA OF BIOVAR ELTOR - PRODUCER OF PROTECTIVE O1 ANTIGEN | 2010 |
|
RU2425867C1 |
| AVIRULENT STRAIN OF Vibrio cholerae KM 262 BACTERIA OF SEROVAR OGAWA OF BIOVAR ELTOR - PRODUCER OF PROTECTIVE O1 ANTIGEN | 2010 |
|
RU2425868C1 |
| METHOD OF PRODUCING AND RECORDING MARKED BACTERIOPHAGES IN CHOLERA GERM MODEL | 2009 |
|
RU2422520C1 |
| RECOMBINANT PLASMID EXPRESSING VIBRIO CHOLERAE CLONED CEF GENE (VARIANTS) AND ESCHERICHIA COLI STRAIN-PRODUCER OF VIBRIO CHOLERAE CEF (VARIANTS) | 2005 |
|
RU2313577C2 |
| STRAIN OF CULTURED ANIMAL HYBRID CELLS MUS. MUSCULUS - PRODUCER OF MONOCLONAL ANTIBODIES TO THE MEMBRANE PROTEIN COMMON TO TYPICAL AND ATYPICAL SEROGROUP 01 CHOLERA VIBRIOS | 2022 |
|
RU2785463C1 |
| STRAIN OF BACTERIA VIBRIO CHOLERAE ELTOR INABA - A PRODUCER OF @@@-AMYLASE | 0 |
|
SU1726514A1 |
