FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, in particular to a recombinant plasmid DNA pET31b-pHLIP. Said plasmid DNA encodes an amino acid sequence of a recombinant pH-dependent incorporating peptide and provides its synthesis in a fused protein with ketosteroid isomerase. Invention also relates to a method for obtaining said peptide. Present method involves the transformation of Escherichia coli cells of plasmid DNA pET31b-pHLIP, growing of transformed cells in a medium with ampicillin, induction of expression, subsequent cultivation, separation of cells by centrifugation, washing of inclusion bodies, their dissolution in a buffer with the addition of urea, purification by metal-chelate chromatography, subsequent separation of the peptide from ketosteroid isomerase and 6 histidine residues by using bromocyan.
EFFECT: present invention allows obtaining a recombinant pH-dependent incorporating peptide.
2 cl, 9 dwg, 5 ex
| Title |
Year |
Author |
Number |
| METHOD OF PRODUCING RECOMBINANT ANTIMICROBIAL PEPTIDE UBI18-35, RECOMBINANT PLASMID DNA pET31b-2xUBI18-35 AND PRODUCER-PRODUCER OF ESCHERICHIA COLI BL21 ROSETTA DE3 pLusS/pET31b-2HUBI18-35 ANTIMICROBIAL PEPTIDE UBI18-35 |
2018 |
- Pershina Aleksandra Gennadevna
- Efimova Lina Viktorovna
- Ashcheulova Darya Olegovna
|
RU2698037C1 |
| PLASMID VECTOR pE-Trx-Aur, STRAIN ESCHERICHIA COLI FOR EXPRESSION OF ANTIMICROBIAL PEPTIDE AURELIN AND METHOD OF SAID PEPTIDE OBTAINING |
2009 |
- Balandin Sergej Vladimirovich
- Finkina Ekaterina Ivanovna
- Kokrjakov Vladimir Nikolaevich
- Ovchinnikova Tat'Jana Vladimirovna
|
RU2412999C1 |
| RECOMBINANT PLASMID DNA pEst877 DETERMINING EXPRESSION OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE Psychrobacter cryohalolentis K5 ON CELL SURFACE Escherichia coli, AND BACTERIA STRAIN Escherichia coli BL21 (DE3) pLysS/pEst877-PRODUCER OF POLYPEPTIDE WITH ACTIVITY OF ESTERASE PSYCHROBACTER CRYOHALOLENTIS K5 ON CELL SURFACE |
2013 |
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Novototskaja-Vlasova Ksenija Aleksandrovna
- Rivkina Elizaveta Mikhajlovna
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2526213C1 |
| RECOMBINANT PLASMID DNA pMALTEV-legumain, ENCODING POLYPEPTIDE HAVING ANTIGENIC PROPERTIES OF PROTEIN LEGUMAIN Opisthorchis felineus, AND STRAIN E.coli BL 21(DE3)pLysS-pMALTEV-legumain - PRODUCENT OF RECOMBINANT POLYPEPTIDE HAVING ANTIGENIC PROPERTIES OF PROTEIN LEGUMAIN Opisthorchis felineus |
2012 |
- L'Vova Marija Nikolaevna
- Mordvinov Vjacheslav Alekseevich
- Ponomareva Evgenija Pavlovna
- Sivkov Anton Jur'Evich
- Razumov Ivan Alekseevich
- Katokhin Aleksej Vadimovich
|
RU2496876C1 |
| PLASMID VECTOR pET-His8-TrxL-Acip1 STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET-His8-TrxL-Acip1 FOR EXPRESSION OF ANTIMICROBIAL PEPTIDE ACIPENSINA-1 AND METHOD OF PRODUCING SAID PEPTIDE |
2015 |
- Balandin Sergej Vladimirovich
- Panteleev Pavel Valerevich
- Bolosov Ilja Aleksandrovich
- Shamova Olga Valerevna
- Kokrjakov Vladimir Nikolaevich
- Ovchinnikova Tatjana Vladimirovna
|
RU2580031C2 |
| RECOMBINANT PLASMID DNA pFK2 PROVIDING SYNTHESIS OF RECOMBINANT PEPTIDE BEING HUMAN KAPPA CASEIN FRAGMENT ANALOGUE, METHOD FOR PREPARING RECOMBINANT PEPTIDE, AND RECOMBINANT PEPTIDE, HUMAN KAPPA CASEIN FRAGMENT ANALOGUE EXHIBITING APOPTOTIC ACTIVITY IN RELATION TO MALIGNANT CELLS |
2009 |
- Tikunova Nina Viktorovna
- Semenov Dmitrij Vladimirovich
- Babkina Irina Nikolaevna
- Kuligina Elena Vladimirovna
- Koval' Ol'Ga Aleksandrovna
- Fomin Aleksandr Sergeevich
- Matveeva Vera Aleksandrovna
- Matveev Andrej Leonidovich
- Matveev Leonid Ehduardovich
- Rikhter Vladimir Aleksandrovich
|
RU2401307C1 |
| RECOMBINANT PLASMID DNA pЕ-Trx-Lc-def, Escherichia coli STRAIN FOR EXPRESSION OF ANTIMICROBIAL PEPTIDE OF LENTIL DEFENSIN Lens culinaris AND METHOD FOR PRODUCING SAID PEPTIDE |
2010 |
- Balandin Sergej Vladimirovich
- Finkina Ekaterina Ivanovna
- Ovchinnikova Tat'Jana Vladimirovna
|
RU2456345C1 |
| RECOMBINANT PLASMID DNA pE-His8-TrxL-Ar2 ENCODING FUSION PROTEIN COMPRISING ANTIMICROBIAL SEA ANNELID WORM Arenicola marina PEPTIDE ARENICIN AND STRAIN Escherichia coli BL21(DE3)/pE-His8-TrxL-Ar2 AS PRODUCER OF ARENICIN-CONTAINING FUSION PROTEIN |
2006 |
- Balandin Sergej Vladimirovich
- Kokrjakov Vladimir Nikolaevich
- Ovchinnikova Tat'Jana Vladimirovna
|
RU2316590C1 |
| RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE |
2015 |
- Petrovskaya Lada Evgenevna
- Shingarova Lyudmila Nikolaevna
- Kryukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Zlobinov Aleksandr Vladimirovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2606014C1 |
| RECOMBINANT PLASMID DNA PSMT3_HCRG21 ENCODING A HYBRID PROTEIN SMT3-HCRG21, BACTERIAL STRAIN ESCHERICHIA COLI BL21(DE3)/PSMT3_HCRG21 - PRODUCER OF HCRG21 ANALGESIC PEPTIDE AND METHOD OF PRODUCING RECOMBINANT HCRG21 ANALGESIC PEPTIDE |
2022 |
- Tereshin Mikhail Nikolaevich
- Stepanenko Vasilii Nikolaevich
- Leichenko Elena Vladimirovna
- Kozlovskaia Emma Pavlovna
- Podvolotskaia Anna Borisovna
- Tekuteva Liudmila Aleksandrovna
- Kozlov Sergei Aleksandrovich
|
RU2798545C1 |
