FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. In one embodiment, the output (g) of the copies of the nucleic acid to be amplified at the end of at least one of the cycle passes is less than 80% of the copies of the nucleic acid available at the beginning of this passage, and at least one of the cycles of the cycle duration of exposure (tA) is less than one second. In another embodiment, the amount (k) of the polymerase chain reaction cycle passes is greater than 45 and/or at least one of the passes the cycle time tc less than 20 seconds.
EFFECT: invention relates to a method for amplifying nucleic acids by means of a polymerase chain reaction in which a cycle consisting of denaturing, annealing and elongation steps is repeatedly performed.
29 cl, 6 dwg, 3 ex, 3 tbl
