FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, namely to a method for indirect control of completeness of FMD virus antigen inactivation using nested reverse transcriptase polymerase chain reaction (hereinafter – RT PCR), followed by electrophoresis of amplicons in agarose gel. The completeness of FMD virus antigen inactivation is determined by a nested RT PCR, using two original primer systems: 5'NTR-F1 and 3D-R1 (amplification of a genome section of FMD virus with a size of 7289 n. p.), 5'NTR-F2 and 3D-R2 (amplification of a genome section of FMD virus with a size of 7244 n. p.). The detection of the results of indirect control of completeness of FMD virus inactivation is performed in a stream of ultraviolet light with a wavelength of 312 nm after horizontal electrophoresis. In the presence of fluorescent amplicons obtained in PCR2, using the second original primer system, the sample is virulent, in the absence of fluorescent PCR2-products, the sample is inactivated.
EFFECT: proposed invention allows for simultaneous study of up to 89 samples of FMD virus antigen, allows for indirect determination of completeness of FMD virus antigen inactivation 7.2 times faster compared to the cultural method and with high degree of reliability.
7 cl, 3 dwg, 12 tbl, 6 ex
