METHOD AND KIT FOR SYNTHESIS OF NUCLEAR ACIDS HAVING NUCLEOTIDE SEQUENCE WHEREIN COMPLEMENTARY NUCLEOTIDE SEQUENCES ARE ALTERNATELY BONDED IN ONE CHAIN Russian patent published in 2005 - IPC

FIELD: biotechnology, in particular pharmaceutical industry and medicine.

SUBSTANCE: method and kit for nuclear acid synthesis are disclosed. Said kit includes DNA-polymerase, catalyzing synthesis of complementary chain according to replacement mechanism, substrate therefore, two inner primer, and two oligonucleotide. Claimed method includes mixing of said kit with nuclear acid sample and mixture incubation at temperature sufficient for saving enzymatic activity of DNA-polymerase and stable coupling of sequence bases of inner primers with sequences being complementary thereto. Method of present invention makes it possible to obtain nuclear acid with nucleotide sequence wherein complementary nucleotide sequences are alternately bonded in one chain.

EFFECT: inexpensive method with increased synthesis specificity.

9 cl, 18 dwg, 8 ex