METHOD OF BIOLOGICAL MONITORING OF ECOSYSTEM STATUS IN KOZMINO BAY WATER AREA WITH USE OF MARINE HYDROBIONTS AS TEST OBJECTS Russian patent published in 2018 - IPC G01N33/18 

FIELD: ecology.

SUBSTANCE: invention relates to ecology, namely to a method for assessing the state of marine ecosystems in zones affected by pollution sources using marine hydrobionts cultivated in a water plantation as test objects, and/or hydrobionts living in natural settlements of the Kozmino Bay water area at various intervals. To this end a coastal scallop Mizuhopecten yessoensis, a gray sea urchin Strongylocentrotus intermedius, a black sea urchin Mesocentrotus nudus or meroplankton are used as hydrobionts. After sampling the tissues of the cultured scallop Mizuhopecten yessoensis, histomorphological analysis is carried out and the lipofuscin content in the tissue cells is determined, the size of the lipofuscin granules should be from 1 to 3 mcm, conglomerates not more than 12 mcm, and the occupied area not more than 0.1 % of the area of the histological section. Also determine the size of the coastal scallop Mizuhopecten yessoensis, cultivated on the plantation in the water area of the bay, or the coastal scallop Mizuhopecten yessoensis, inhabiting natural settlements, and compare the obtained data with the normative indicators or compare it with the normative indicators that form on the basis of the average indicators for 3–10 years for individuals living in natural settlements. In addition, the mass and size of gray sea urchins of Strongylocentrotus intermedius or black sea urchins Mesocentrotus nudus are determined or the quantitative composition of meroplankton is determined and compared with the normative indices, that are formed on the basis of the average indicators for 3–10 years. When changing the quantitative, size and sex and age indicators of marine hydrobionts in comparison with the normative indicators, the conclusion is made about the influence of pollution sources on the state of marine ecosystems and on the ecological state of the Kozmino Bay water area.

EFFECT: invention provides an effective monitoring of the state of the aquatic environment and possible consequences of salvo or chronic pollution.

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