FIELD: biotechnology.
SUBSTANCE: method for diagnosing the polymorphism of the CARD15 gene, including a single-tube polymerase chain reaction (PCR) and two pairs of primers (CARD15_Rev_A_NEW-5’-GGC CAG GGT ACA AGG GAA AG, CARD15_For_A_NEW-5’ -GGA AAT TGA GAA ACT CAG CCA GCA, CARD15_Rev_T_NEW-5’ -CAG AGC AAG AGT CTG GTA TGC A, CARD15_For_T_NEW-5’ -GCA CGG TGA TTC ATG AGC TG) for the amplification of two different alleles of single nucleotide polymorphism: fragment of the gene with A (562 bp) allele is associated with a decreased content of somatic cells, and the region of the T (282 bp) allele is associated with an increased content of somatic cells in the cow's milk.
EFFECT: invention is applicable in the selection of cattle and veterinary medicine.
1 cl, 1 dwg, 1 ex
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