KIT FOR MULTIPLEX IMMUNOCHEMICAL ANALYSIS OF ANTIBODIES AND ANTIGENS IN BLOOD PREPARATIONS Russian patent published in 2019 - IPC G01N33/543 

Abstract RU 2686490 C1

FIELD: medicine.

SUBSTANCE: invention relates to sets for immunochemical analysis of antibodies and antigens in blood preparations and can be used in medical diagnostics. Disclosed is a kit for multiplex immunochemical analysis of antibodies and antigens in blood preparations, comprising a device in the form of a comb-like substrate with a set of reagents discretely deposited on the surface of substrate teeth of infectious disease markers and additionally blocked with nonspecific natural or synthetic polymers; an analysis container comprising several rows of isolated cells configured to insert each substrate wave into a separate cell of the bath; solutions for dilution of sample and washing; conjugate for detection; built-in positive control and negative control. Capture reagents contain antigens and antibodies to markers of one or more agents of infectious diseases; immobilized on each substrate wave; conjugate for detection contains a multicomponent composition containing several types of detector antibodies against human immunoglobulins and against detectable antigens associated with catalytically active gold sols; positive controls include assessment of operability of all specific conjugate components, are located on separate segments of immunochip and include an area for monitoring operability of anti-species detector antibodies, a region for monitoring specific detecting antibodies to each detectable antigen and a negative control region free from specific proteins; developer of the immunochip contains dry tableted and liquid components and is equipped with an amplifier and a color stabilizer of developed immunochips.

EFFECT: invention provides higher information value of multiplex analysis and diagnostics of early stages of infection ensured by simultaneous detection in samples of antibodies and antigens of interest.

5 cl, 4 dwg, 1 tbl, 2 ex

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RU 2 686 490 C1

Authors

Poltavchenko Aleksandr Georgievich

Nechitajlo Oleg Vyacheslavovich

Filatov Pavel Vladimirovich

Ersh Anna Vasilevna

Dates

2019-04-29Published

2018-06-01Filed