FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a method of producing an anti-FcRH5 antibody which binds to an isoform c-specific region of the extracellular domain of FcRH5c and binds insignificantly to another Ig-like domain of FcRH5. Method involves immunising a mouse with an antigen, recovering lymphocytes from a mouse and fusing with myeloma cells to obtain hybridoma and collecting the anti-FcRH5 antibody from the hybridoma. Also disclosed is an anti-FcRH5 antibody prepared by said method and an immunoconjugate based thereon. Polynucleotide and a host cell for producing the antibody according to the invention are described. Antibody and immunoconjugate of the invention are used to produce pharmaceutical compositions for treating FcRH5-positive cancer, in methods of treating patients suffering FcRH5-positive cancer, inhibiting proliferation of FcRH5-positive cells. Also disclosed is a method of detecting FcRH5 in a sample and a method of detecting FcRH5-positive cancer.
EFFECT: invention can be used in diagnosing and treating cancer.
68 cl, 25 dwg, 4 tbl
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