METHOD FOR DETERMINING ANTIBIOTIC PROPERTIES OF MATERIALS Russian patent published in 2019 - IPC C12Q1/18 C12N1/20 C12R1/00 

FIELD: measurement technology.

SUBSTANCE: invention relates to bio-measuring technologies. Method for determining the antibiotic properties of materials is proposed. Method comprises incubating the test strain Rhodotorula sp. VKM Y-2993D in an amount of from 5×10⁵ to 5×10⁶ viable cells per ml in a liquid nutrient medium pH 6.6–7.4 for 10–20 hours at 15–25 °C in the presence of tested materials – hydrocarbons and in their absence. Measure the pH, the intensity of light scattering at a wavelength of from 500 to 950 nm (Iod) and the optical density of the samples at a wavelength of from 230 to 320 nm (Auv). Total degree of activation or inhibition (+/-) of the test microorganisms activity by the tested materials is determined using formula: ε_S=(ε_Iod+ε_Auv+ε_pH)/3, where ε_pH, ε_Iod and ε_Auv determined using formulas: ε_Y=100×(ΔYt-ΔYc)/ΔYc where ΔY=Ye-Yb are differences in pH, Iod, or Auv values recorded at the beginning (Yb) and at the end (Ye) of the incubation of test samples in the presence of test materials (ΔYt) and in their absence (ΔYc).

EFFECT: method provides an objective determination of the antibiotic properties of materials, including hydrocarbons.

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