METHOD FOR EVALUATING PRO- AND ANTIMICROBIAL PROPERTIES OF SAMPLES Russian patent published in 2019 - IPC C12Q1/18 C12N1/20 C12R1/225 

FIELD: measurement technology.

SUBSTANCE: invention relates to bio-measuring technologies. Method for evaluating the pro- and antimicrobial properties of samples is proposed. Method comprises incubating the test microorganisms Lactobacillus sp. in quantities of 5×10⁵ to 5×10⁶ viable cells per ml in a liquid nutrient medium pH 6.6–7.4 for 4–8 hours at a temperature of from 20 to 45 °C in the presence of test samples and in their absence. Measure pH, redox potential (E) and electrical conductivity (X) of the samples. Total degree of activation or inhibition (+/-) of the test microorganisms activity by the tested samples is determined using formula: ε_S=(ε_pH+0.4ε_E+0.6ε_X)/2, where ε_pH, ε_E and ε_X determined by the formulas ε_Y=100×(ΔYt-ΔYc)/ΔYc where ΔY=Ye-Yb are differences in pH, E or X values recorded at the beginning (Yb) and at the end (Ye) of the incubation of test samples in the presence of test samples (ΔYt) and in their absence (ΔYc).

EFFECT: method provides an objective assessment of the pro- and antimicrobial properties of samples, including those containing actively light-absorbing or light-scattering substances and material.

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