FIELD: biotechnology.
SUBSTANCE: invention relates to production of recombinant human butyrylcholinesterase (BuChE). Task is solved by creating a genetic construct - recombinant plasmid DNA pFUSE-MARX-29-PRAD-F2A/BChE-14 containing a nucleotide sequence of a synthetic gene of modified human butyrylcholinesterase coding a version of butyrylcholinesterase with replacement of sequence 284-TPLSV-288 with sequence 284-HTIHG-288, providing production of modified enzyme rhBChE-14, capable of reactivation after covalent inhibition by paraoxone and not subject to aging, a nucleotide binding sequence with a nuclear matrix (MAR X-29), which enables to increase production of the target protein due to both increase in the level of transcription of the gene located in close proximity, and due to higher stability of the ribosome-transcript complex.
EFFECT: invention can be used in medicine in therapy of organophosphorus toxins, for therapy of narcotic substance poisonings of cocaine type, for therapy of consequences of anesthesia in people with endogenous Buchester deficiency.
1 cl, 3 dwg, 1 tbl, 4 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA pPicZαA/BChE-14 CONTAINING HUMAN MODIFIED BUTYRYLCHOLINESTERASE GENE FOR EXPRESSION OF BUTYRYLCHOLINESTERASE GENE IN METHYLOTROPHIC YEAST PICHIA PASTORIS FOR THERAPY OF ORGANOPHOSPHOROUS TOXINS POISONING |
2016 |
- Bobik Tatyana Vladimirovna
- Gabibov Aleksandr Gabibovich
- Mokrushina Yuliana Anatolevna
- Smirnov Ivan Vitalevich
- Terekhov Stanislav Sergeevich
|
RU2711939C2 |
| RECOMBINANT PLASMID DNA OF PFUSE/SCFV47FC, INTENDED FOR PRODUCING SINGLE-CHAIN ANTIBODY FUSED WITH HUMAN IMMUNOGLOBULIN CONSTANT FRAGMENT INACTIVATING PARAOXONE PESTICIDE |
2019 |
- Mokrushina Yuliana Anatolevna
- Pipiya Sofiya Oniseevna
- Golovin Andrej Viktorovich
- Smirnov Ivan Vitalevich
- Gabibov Aleksandr Gabibovich
|
RU2732835C1 |
| METHOD FOR PRODUCTION OF CHEMICALLY POLYSIALYLATED RECOMBINANT HUMAN BUTYRYLCHOLIN ESTERASE |
2012 |
- Ilyushin Denis Grigorevich
- Belogurov Aleksej Anatolevich
- Ertle Oksana Mikhajlovna
- Gabibov Aleksandr Gabibovich
- Surkov Kirill Gennadievich
- Ponomarenko Natalya Aleksandrovna
- Patrik Masson
- Smirnov Ivan Vitalevich
|
RU2645458C2 |
| RECOMBINANT FUSED PROTEIN |
2019 |
- Maksimyak Sergej Petrovich
- Al-Shekhadat Ruslan Ismailovich
|
RU2732795C1 |
| GENETIC CASSETTE CONTAINING CODON-OPTIMIZED NUCLEOTIDE SEQUENCES OF GENES HEXA AND HEXV, AND PHARMACEUTICAL COMPOSITION FOR TREATING TAY-SACHS DISEASE |
2019 |
- Shajmardanova Alisa Almazovna
- Chulpanova Darya Sergeevna
- Soloveva Valeriya Vladimirovna
- Rizvanov Albert Anatolevich
|
RU2723187C1 |
| GENETIC CASSETTE CONTAINING CODON-OPTIMIZED NUCLEOTIDE SEQUENCES OF TRAIL, PTEN AND IFNβ-1 GENES, AND PHARMACEUTICAL COMPOSITION FOR THE TREATMENT OF ONCOLOGIC DISEASES |
2020 |
- Chulpanova Darya Sergeevna
- Soloveva Valeriya Vladimirovna
- Rizvanov Albert Anatolevich
|
RU2757502C1 |
| RECOMBINANT PLASMID pOK-DsRed2, CODING OCT4 AND KLF4 HUMAN PROTEINS AND FLUORESCENT PROTEIN DsRed2, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS |
2011 |
- Medvedev Sergej Petrovich
- Shevchenko Aleksandr Igorevich
- Pokushalov Evgenij Anatol'Evich
- Zakijan Suren Minasovich
|
RU2495125C2 |
| RECOMBINANT PLASMID POL-DSRED2, CODING OCT4 AND LIN28 HUMAN PROTEINS AND FLUORESCENT PROTEIN DSRED2, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS |
2011 |
- Medvedev Sergej Petrovich
- Shevchenko Aleksandr Igorevich
- Pokushalov Evgenij Anatol'Evich
- Zakijan Suren Minasovich
|
RU2495126C2 |
| GENETIC CONSTRUCT pA21/Fab, FOR PRODUCING A SINGLE-CHAIN ANTIBODY FUSED WITH A CONSTANT HUMAN IMMUNOGLOBULIN FRAGMENT INTERACTING STEREOSELECTIVELY WITH ORGANOPHOSPHORUS COMPOUNDS |
2017 |
- Mokrushina Yuliana Anatolevna
- Golovin Andrej Viktorovich
- Pipiya Sofiya Oniseevna
- Smirnov Ivan Vitalevich
- Gabibov Aleksandr Gabibovich
|
RU2693553C1 |
| MTS GENE, MUTATIONS OF THIS GENE AND METHODS OF DIAGNOSIS OF MALIGNANT TUMORS USING MTS GENE SEQUENCE |
1995 |
|
RU2164419C2 |
