FIELD: biotechnologies.
SUBSTANCE: plasmid genetic structure pOK-DsRed2 is produced, being built on the basis of a plasmid vector pIRES (Clontech), where fragments of cDNA of human genes OCT4 and KLF4 are placed, being connected with a nucleotide sequence coding P2A-peptide and gene cDNA, coding fluorescent protein DsRed2. Transcription of a single mRNA OCT4-F2A-KLF4-IRES-DsRed2 is carried out from constitutive promotor p CMV IE, providing for high level of mRNA lifelength.
EFFECT: availability of F2A and IRES sequences makes it possible to simultaneously translate proteins OCT4, KLF4 and DsRed2 from one mRNA molecule.
2 cl, 1 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID POL-DSRED2, CODING OCT4 AND LIN28 HUMAN PROTEINS AND FLUORESCENT PROTEIN DSRED2, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS | 2011 |
|
RU2495126C2 |
| RECOMBINANT PLASMID pSM-ZsGreen, CODING HUMAN PROTEINS SOX2 AND C-MYC AND FLUORESCENT PROTEIN ZsGreen, INTENDED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS | 2011 |
|
RU2477314C1 |
| RECOMBINANT PLASMID pSN-ZsGreen, CODING SOX2 AND NANOG HUMAN PROTEINS AND FLUORESCENT PROTEIN ZsGreen, DESIGNED TO PRODUCE INDUCED PLURIPOTENT HUMAN STEM CELLS | 2011 |
|
RU2495124C2 |
| RECOMBINANT PLASMID pAd-SM, CODING HUMAN PROTEINS SOX2 AND C-MYC, BEING BASE FOR PRODUCTION OF VIRULENT ADENOVIRUSES DESIGNED FOR PRODUCTION OF INDUCED PLURIPOTENT HUMAN CELLS | 2012 |
|
RU2495127C2 |
| METHOD OF PRODUCING INDUCED PLURIPOTENT STEM CELLS FROM FIBROBLASTS OF PATIENTS WITH HUNTINGTON'S DISEASE | 2011 |
|
RU2458983C1 |
| RECOMBINANT PLASMID DNA pBi101-IL18 ENCODING HUMAN INTERLEUKIN-18 SYNTHESIS IN TRANSGENIC PLANTS | 2005 |
|
RU2302460C1 |
| METHOD OF USING STEM CELLS IN TISSUE REPLACEMENT THERAPY | 2009 |
|
RU2422514C1 |
| METHOD OF GROWING TRANSGENIC CARROT PLANTS, WHICH PRODUCE HUMAN INTERLEUKIN-10 | 2007 |
|
RU2374321C2 |
| RECOMBINANT PLASMID DNA pSNCA-C-3XFLAG-2XST-DONOR PROVIDING STABLE DOXYCYCLINE-DRIVEN EXPRESSION OF ALPHA-SYNUCLEIN CHIMERIC PROTEIN IN HUMAN CELL CULTURES | 2022 |
|
RU2795156C1 |
| METHOD OF PROVIDING SAFETY OF APPLICATION OF PLURIPOTENT STEM CELLS IN TISSUE-SUBSTITUTING THERAPY BY MEANS OF ARTIFICIAL CHROMOSOMES | 2012 |
|
RU2548819C2 |
