FIELD: molecular biology, biotechnology, genetic engineering. SUBSTANCE: invention relates to amino acid sequences of α-and β-subunits of polypeptide with activity of nitrile hydratase and DNA fragments encoding this polypeptide. Also, invention relates to recombinant plasmid DNA pNHJ20L encoding polypeptide eliciting properties of nitrile hydratase, transformer containing recombinant plasmid DNA and method of nitrile hydratase preparing. DNA fragment encoding polypeptide with nitrile hydratase activity is inserted into plasmid vector, strain E. coli is transformed using plasmid pNHJ20L and the obtained strain TG1/pNHJ20L (FERM BP-2778) is able to produce polypeptide with properties of nitrile hydratase. EFFECT: increased yield of polypeptide of strain as compared with that in microorganisms used in common practice. 5 cl, 4 dwg
| Title |
Year |
Author |
Number |
| DNA FRAGMENT ENCODING POLYPEPTIDE EXHIBITING ACTIVITY OF NITRILE HYDRATASE, RECOMBINANT PLASMID DNA ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE PROPERTIES, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE SHOWING NITRILE HYDRATASE PROPERTIES, METHOD OF PREPARING POLYPEPTIDE SHOWING NITRILE HYDRATASE PROPERTIES |
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- Terukhiko Beppu[Jp]
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- Terukhiko Beppu[Jp]
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- Makato Nisijama[Jp]
|
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|
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- Terukhiko Beppu[Jp]
- Khideaki Jamada[Jp]
- Toru Nagasava[Jp]
- Suekharu Khorinouti[Jp]
- Makoto Nisijama[Jp]
|
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- Kendzi Mitsusima[Jp]
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- Takajasu Sonojama[Jp]
|
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- Takajama Masanori
- Kojama Nobuto
- Kato Ikunosin
- Sakai Takesi
|
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- Tosio Miyake
- Yasuo Khibino
- Eiti Kobayasi
- Ken Vatabe
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- Rejko Matsumoto
- Kazuo Vatanabe
- Naganori Numao
|
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- Startsev Veniamin Aleksandrovich
- Startsev Innokentij Aleksandrovich
- Startseva Julija Vladimirovna
- Sopova Julija Viktorovna
- Zadorskij Sergej Pavlovich
|
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- Sukhanova L.L.
- Alatortseva G.I.
- Gol'Tsov V.A.
- Alatortsev V.E.
- Zverev V.V.
- Poletaeva N.N.
- Blinov V.M.
- Grinev A.A.
- Krasnykh V.N.
|
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- Osval'D Shteffen
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|
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