METHOD FOR CULTIVATION OF BLACK SEA RED ALGAE GELIDIUM SPINOSUM (GREV.) BORN. ET THUR (RHODOPHYTA) Russian patent published in 2019 - IPC C12N1/12 A01G33/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and can be used in cultivation of Black Sea red agarous algae Gelidium spinosum (Grev.) Born. et Thur in coastal systems of engineering type. Method for cultivation of Black Sea red alga Gelidium spinosum (Grev.) Born. et Thur (Rhodophyta) provides weekly cultivation, including phases of "rest" in an aquarium at temperature of 10–12 °C and day illumination 0.2–0.5 kl. Nutrient medium is prepared on the basis of filtered Black Sea inlet, brought to salinity of 26 ‰, containing KNO₃, KH₂PO₄⋅3H₂O, FeSO₄⋅7H₂O pre-boiled in distillate together with Na₂ EDTA, MnCl₂x4H₂O and MgSO₄x7H₂O in given amounts with further inoculation of nutrient medium with helium fragments with initial density of 2–2.5 kg/m². Helidium fragments are grown in cultivators at illumination of 18–20 kl in the mode "16 hours - day, 8 hours - night", at temperature of nutrient medium - 15–19 °C-February, March, November; 19–23 °C-April, May, September and October; and in summer months -25–27 °C its further retention specified time and decrease to end of cycle. Method includes bubbling of medium with compressed air and maintenance of required pH value of medium by adding carbon dioxide at rate of 25–30 g per kg of algae a day with subsequent flow of nutrient medium through cultivators, harvesting and sampling for subsequent cycles, the most complete and clean from fouling algae. At the same time flow of nutrient medium through cultivators is set from 1–1.7 to 1.6–2 volumes a day at the beginning and at the end of the cycle, respectively, with a daily increase in flow by 10–5%. From the middle to the end of the next cycle the average temperature characteristic for the given period is maintained, and through the aquarium in the mode of "rest of culture" - constant channel up to 0.05 of volume a day.

EFFECT: invention increases output of R-phycoerythrin.

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