METHOD FOR OBTAINING BIOMASS OF CYLINDROTHECA CLOSTERIUM DIATOM WITH HIGH FUCOXANTHIN CONTENT Russian patent published in 2018 - IPC A01G33/00 C12N1/12 

FIELD: biotechnology.

SUBSTANCE: method provides a storage mode of cultivation, namely the actively dividing culture of Cylindrotheca closterium diatom, taken at the linear stage of growth, with an initial density of 0.1–0.2 g of dry matter per 1 liter of culture, are cultured for 10-12 days in photobioreactors in the form of cultivators of plane-parallel type with a thickness of the illuminated layer of 5 cm, on a modified nutrient medium prepared on the basis of sterilized sea water, g⋅l^-1, with the following composition: NaNO₃ - 7.50, Na₂SiO₃×9H₂O - 3.00, NaH₂PO₄×2H₂O - 0.50, Na₂EDTA - 0.872, FeSO₄×7H₂O - 0.63, NaMoO₄×H₂O - 0.063, CuSO₄×5H₂O - 0.1, ZnSO₄×7H₂O - 0.22, CoCl₂×6H₂O - 0.1, MnCl₂×4H₂O - 0.18.

EFFECT: method makes it possible to increase the content of fucoxanthin in the biomass of Cylindrotheca closterium diatom.

1 cl, 3 dwg, 1 tbl, 2 ex