METHOD FOR DETERMINING CONTENT OF CARAGEENAN IN MEAT PRODUCTS Russian patent published in 2019 - IPC G01N33/12 

FIELD: veterinary medicine.

SUBSTANCE: method relates to the field of veterinary and sanitary examination of livestock products and represents a method for determining content of carrageenan in meat products, involving sampling, characterized in that a sample of the analyzed product is sampled, equal to 0.2 g, is placed in a test tube with 2 ml of 10 % potassium hydroxide solution, heated in a boiling water bath at 100 °C for 30 minutes with periodic stirring, cooled to room temperature, adding 8 ml of cooled 95° ethyl alcohol, intensively mixed and placed in a freezer at −10 °C – −20 °C for 1 hour, centrifuged at 3000 rpm for 10 minutes, precipitate is dried in a drying cabinet at 50–60 °C is dissolved at 100°C in 1 ml of distilled water, the solution is cooled to room temperature, 1 ml of 50 % solution of freshly received whole human saliva is added, the liquid is stirred and pH is adjusted to 7.3, solution is incubated at 37 °C for 15 minutes, reiterated 8 ml 95° ethyl alcohol, placed in a freezer at −10°C – −20 °C for 60 minutes, the precipitate is centrifuged at 3000 rpm for 10 minutes, supernatant is decanted, the test tube is dried in a drying cabinet at 50–60 °C, carageenan precipitate is separated from the bottom of the test tube and weighed, the amount of carrageenan is converted to 100 g of the meat product.

EFFECT: use of the declared method enables reducing labour intensity, energy intensity and duration of investigations, and improving accuracy of research results.

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