METHOD OF DETERMINING TOTAL CONTENT OF PHENOL COMPOUNDS IN PLANT OBJECTS Russian patent published in 2019 - IPC G01N33/00 A01G7/00 

FIELD: biochemistry of plants.

SUBSTANCE: invention relates to physiology and biochemistry of plants and can be used in determining content of phenol compounds (PC) in plant objects. Method involves milling vegetal raw materials, extraction in alcohol while stirring, subsequent centrifugation, adding Folin-Ciocalteu reagent and sodium carbonate to the extract. Raw material with weight of 0.05 g is ground with 1.5 cm³ 96 % ethanol, extraction of phenol compounds is carried out for 45 minutes at 45 °C with periodic stirring (every 15 minutes) and subsequent centrifugation for 2 minutes at rotation speed of 16,000 rpm, from obtained extract samples are taken, with volume of 0.075 cm³, adding to them at 0.075 cm³ Folin-Ciocalteu reagent diluted 5-fold is mixed, after 3 min 0.15 cm³ 20 % solution of sodium carbonate and 1.2 cm³ distilled water, closed with a cover, stirred and left at room temperature, and after 1 hour, the optical density of the formed tungsten blue is measured at wavelength 725 nm, the length of the optical path is 1 cm. Total content of PC is expressed in mg-equivalent gallic acid per g raw material weight.

EFFECT: use of the invention enables to develop a safe, cost-effective method of determining total content of PC in plant objects using standard equipment for laboratory analysis, without using methanol, while reducing extraction time.

1 cl, 3 tbl, 2 ex