METHOD FOR QUANTITATIVE DETERMINATION OF GLYCINE IN BIOLOGICAL MEDICINAL PREPARATIONS BY HYDROPHILIC HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY Russian patent published in 2019 - IPC G01N30/02 

FIELD: meeting human vital needs; physics.

SUBSTANCE: invention refers to public health, in particular to quality control of biological medicinal preparations, and can be used for quantitative determination of glycine in biological medicinal preparations containing glycine as an auxiliary substance. Method for quantitative determination of glycine in biological medicinal preparations by hydrophilic high-performance liquid chromatography is characterized by using SeQuant ZIC-HILIC chromatographic column, spectrophotometric detector at wavelength of 210 nm, isocratic mode of elution with flow rate of mobile phase of 0.8 ml/min at temperature of thermostat of chromatographic column 30 °C and volume of sample 20 mcl, then calculating the results using a regression curve of the relative areas of the glycine peak from the concentration of glycine in standard solutions.

EFFECT: simple and high specificity of the method for quantitative determination of glycine in biological medicinal preparations by hydrophilic high-performance liquid chromatography.

3 cl, 5 dwg, 2 tbl