DETERMINATION OF CARBOHYDRATE STABILIZERS IN BIOLOGICALLY ACTIVE PREPARATIONS Russian patent published in 2024 - IPC G01N30/06 G01N30/64 G01N30/34 

FIELD: analytical chemistry.

SUBSTANCE: disclosed is a method for determining carbohydrate stabilizers in biologically active drugs by ion-exchange high-performance liquid chromatography with pulsed amperometric detection, comprising dilution containing at least one carbohydrate stabilizer in a biologically active drug and initial standard solution with concentration of 1.0 mg/l to concentration of carbohydrate stabilizers from 10 to 50 mcg/ml and from 1 to 50 mcg/ml, respectively, or from 25 to 120 mcg/ml and from 2 to 120 mcg/ml, respectively, the obtained solutions are chromatographed in an isocratic elution mode, where the mobile phase is in form of sodium hydroxide or potassium hydroxide with concentration of 160 mmol/l, and then calculations are carried out.

EFFECT: invention provides a method for determining carbohydrate stabilizers in biologically active drugs by ion-exchange high-performance liquid chromatography (ion-exchange HPLC) with pulsed amperometric detection.

7 cl, 16 dwg, 4 tbl, 12 ex