METHOD FOR QUANTITATIVE DETERMINATION OF ASCORBIC ACID IN MEDICINAL HERBAL PREPARATIONS Russian patent published in 2023 - IPC G01N30/02 

FIELD: analytical chemistry.

SUBSTANCE: invention is related in particular to methods for quantitative determination of ascorbic acid in medicinal herbal preparations. The method for quantitative determination of ascorbic acid in herbal medicinal preparations includes the stage of sample preparation, HPLC chromatography and mathematical calculation of the quantitative content of ascorbic acid in the herbal medicinal preparation. To prepare the test solution, 2.5-5 g of the herbal medicinal product is crushed to a particle size of less than 1 mm and mixed with 100 ml of 0.01 M oxalic acid, centrifuged at 5000 rpm for 5 minutes and filtered. To prepare a standard solution, 10 mg - 20 mg of a standard sample of ascorbic acid is dissolved in 50 ml of 0.01 M oxalic acid, then 5 ml of the resulting solution is transferred into a dark glass volumetric flask with a volume of 25 ml, the volume of the solution of 0.01 M oxalic acid is filled to the mark. The sample in chromatographed in gradient mode. The amount of ascorbic acid in the tested herbal medicinal product is calculated.

EFFECT: increase of sensitivity, accuracy and selectivity of the quantitative determination of ascorbic acid in herbal medicines.

1 cl, 7 dwg, 5 tbl, 3 ex