FIELD: biotechnologies.
SUBSTANCE: invention relates to the biotechnology. Disclosed is a method for species differentiation of pathogenic burkholderia by solid-phase immunoassay. Method involves sensibilization of a plate with a mixture of antibodies specific simultaneously to the agents of glanders and melioidosis, use of a pair of species-specific immunoperoxidase conjugates and paired introduction of the analyzed samples into the wells of the plate. Monoclonal antibodies are obtained by immunizing linear mice with cultures of glanders and melioidosis activators, fusion of immune splenocytes with murine myeloma cells, development of linear mice in the abdominal cavity and purification by liquid chromatography.
EFFECT: method for species differentiation of pathogenic Burdcholderia using monoclonal antibodies is proposed.
1 cl, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DIFFERENTIATION PATHOGENIC AND NONPATHOGENIC BURKHOLDERIA | 2008 |
|
RU2378360C1 |
| METHOD OF IMMUNOELECTROPHORETIC DIFFERENTIATION OF MELIOIDOSIS AND FAP AGENTS | 2008 |
|
RU2366715C1 |
| FLUORESCENT-MARKED OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF GLANDERS AND MELIOIDOSIS AGENTS Bpseudomallei AND Bmallei | 2010 |
|
RU2435860C1 |
| STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-10 - PRODUCER OF MONOCLONAL ANTIBODY 5H/E TO ANTIGEN KDA 200 OF MELIOIDOSIS PATHOGEN | 2014 |
|
RU2570638C1 |
| STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-11 - PRODUCERS OF MONOCLONAL ANTIBODY 6A/G TO ANTIGEN 200 kDA OF MELIOIDOSIS PATHOGEN | 2014 |
|
RU2570634C1 |
| METHOD FOR DIFFERENTIATION OF MALLEUS AND MELIOIDOSIS EXITANTS WITH PRECIPITATING ANTISERUM | 2006 |
|
RU2305557C1 |
| STRAIN OF CULTIVATED HYBRID CELLS OF ANIMAL Mus musculus Bpm Vd-8 - PRODUCER OF MONOCLONAL ANTIBODY 3C/A TO ANTIGEN 200 kDa OF PSEUDOCHOLERA ACTIVATOR | 2014 |
|
RU2555544C1 |
| OLIGONUCLEOTIDE PRIMERS FOR β LACTAMASE GENE DETECTON AND TYPING OF PATHOGENIC BURKHOLDERIA | 2011 |
|
RU2474614C1 |
| METHOD OF MELIOIDOZE ANTIGEN ESTIMATE FOR PROTECTIVITY LEVEL | 2007 |
|
RU2354400C1 |
| OLIGONUCLEOTIDE PROBE FOR IDENTIFICATION OF CAUSATIVE AGENTS OF GLANDERS AND MELIOIDOSIS B. pseudomallei AND B. mallei | 2010 |
|
RU2439159C1 |
