FIELD: medicine.
SUBSTANCE: surface antigenic complex (Ag 8) is detected as a part of microbial cells in antigen-antibody crossed electrophorersis with using goat's immune serum containing antibodies to specified antigen recovered from B Pseudomallei 100 with formamide, with antibody titre "РИД" 1:16-1:32. Upon termination of the electrophoresis session, the plates are looked through. Precipitate peaks observed in gel structure testifies the presence of antigenic complex 8 of pathogenic B pseudomallei and B mallei, while the negative result shows the absence of antigens of pathogenic burkholderia.
EFFECT: invention allows for rapid differentiation of the pathogenic agents of melioidosis and glanders and nonpathogenic for humans burkholderia and closely related microorganisms.
3 dwg, 1 tbl, 2 ex
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STRAIN OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L BPM VD-10 - PRODUCER OF MONOCLONAL ANTIBODY 5H/E TO ANTIGEN KDA 200 OF MELIOIDOSIS PATHOGEN | 2014 |
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Authors
Dates
2010-01-10—Published
2008-06-25—Filed