METHOD OF STORING DNA-CONTAINING PLANT MATERIAL IN A WIDE TEMPERATURE RANGE Russian patent published in 2019 - IPC A01N3/00 

Abstract RU 2703058 C2

FIELD: biotechnology.

SUBSTANCE: invention is aimed at storage of samples of DNA-containing plant material. Method of storing DNA-containing plant material involves preparation of plant material homogenate using a buffer solution based on 75 mcl of a buffer solution per 10 mg of tissue. Homogenate is applied on paper carrier impregnated with the same buffer solution and dried at room temperature. Buffer solution consists of 0.5–4 % of N-laurylsarcosine, 5–20 mM of EDTA, 30–200 mM of Tris(hydroxymethyl)aminomethane and water - the rest.

EFFECT: disclosed method of storing DNA-containing plant material enables to preserve DNA for a long time, does not require special storage conditions, can be used to create DNA-banks of plants.

1 cl, 2 dwg, 3 tbl, 3 ex

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RU 2 703 058 C2

Authors

Khachumov Vladimir Arturovich

Kornienko Igor Valerievich

Faleeva Tatyana Georgievna

Usatov Aleksandr Vyacheslavovich

Dates

2019-10-15Published

2017-12-08Filed