FIELD: biotechnology.
SUBSTANCE: method of removing enterovirus RNA in biological material using decontamination solutions is described. The method includes the stages of obtaining a suspension of biological material by mixing fragments of feces or a smear from the rectum of patients with enterovirus infection with physiological solution in a disposable test tube, adding a decontamination solution while stirring on a vortex and incubating for 30 minutes at a temperature of 22–25°C.
EFFECT: invention expands the arsenal of methods of removing enterovirus RNA in biological material.
4 cl, 12 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| COMPOSITION FOR REMOVING DNA- AND/OR RNA-CONTAINING BIOLOGICAL MATERIAL (VARIANTS) | 2021 |
|
RU2789387C1 |
| METHOD FOR DIFFERENTIAL AMPLIFICATION OF VP1 REGION FRAGMENT OF ENTEROVIRUS GENOME ENTEROVIRUS A AND ENTEROVIRUS B | 2020 |
|
RU2743352C1 |
| METHOD FOR AMPLIFYING THE VP1 REGION OF THE GENOME OF ENTEROVIRUS SPECIES ENTEROVIRUS B | 2021 |
|
RU2774424C1 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-MARKED PROBES FOR ENTEROVIRUS RNA IDENTIFICATION BY REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION WITH HYBRIDISATION FLUORESCENT DETECTION | 2011 |
|
RU2459830C1 |
| METHOD FOR ISOLATION OF INTESTINAL VIRUS RNA FOR ANALYSIS BY RT-PCR METHOD | 2006 |
|
RU2313792C1 |
| METHOD FOR CULTIVATION OF FETAL PORCINE CELLS FOR VIROLOGY | 2021 |
|
RU2795135C2 |
| TEST SYSTEM FOR DETECTION OF THE VIRUS GENOME OF PARAINFLUENZA 3 TYPES AT CATTLE WITH A MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME MODE | 2018 |
|
RU2681473C1 |
| METHOD FOR IDENTIFICATION OF 5Т-HTP GENOME OF ENTEROVIRUSES OF EVI AND EVII GENE GROUP IMPLEMENTING POLYMERASE CHAIN REACTION | 2010 |
|
RU2441917C2 |
| INSTANT DIAGNOSTIC TECHNIQUE FOR ENTEROVIRUS ANTIGENS IN CEREBROSPINAL FLUID | 2012 |
|
RU2486520C1 |
| ENTEROVIRUS SUIS STRAIN FOR DIAGNOSIS OF ENTEROVIRUS GASTROENTERITIS OF SWINES | 0 |
|
SU1328375A1 |
