ENZYME PREPARATION FOR HYDROLYSIS OF PLANT MATERIAL AND A METHOD FOR PRODUCTION THEREOF USING RECOMBINANT PRODUCERS BASED ON PICHIA PASTORIS Russian patent published in 2019 - IPC C12P1/00 C12N9/00 

Abstract RU 2705262 C2

FIELD: biotechnologies.

SUBSTANCE: invention relates to the biotechnology. Disclosed is a method of producing an enzyme preparation by culturing in bioreactors of each of recombinant producers P. pastoris K for xylanase from bacteria Nocardiopsis halotolerans VKM Ac-2519, P. pastoris B for beta-glucanase from bacterium Cellulomonas flavigena VKM As-1137, P. pastoris C for cellulase from bacteria Streptomyces bottropensis VKM As-1755, concentration of culture fluid of producers, obtained concentrate is dried and dried preparations of xylanase, beta-glucanase and cellulase are mixed in ratio 1:1:1.

EFFECT: obtained preparation has xylanase activity of not less than 10,000 units/g; cellulase of not less than 5,000 units/g; beta-glucanase not less than 8,000 units/g; maximal activity is manifested in pH range 5–8.

1 cl, 4 dwg, 2 ex

Similar patents RU2705262C2

Title Year Author Number
STRAIN OF TRICHODERMA LONGIBRACHIATUM TW-14-220 FILAMENTOUS FUNGUS - PRODUCER OF CELLULASES, BETA-GLUCANASES AND XYLANASES FOR FEED PRODUCTION AND A METHOD FOR OBTAINING A FEED COMPLEX ENZYME PREPARATION 2017
  • Sinitsyn Arkadij Pantelejmonovich
  • Tsurikova Nina Vasilevna
  • Kostyleva Elena Viktorovna
  • Veselkina Tatyana Nikolaevna
RU2654564C1
STRAIN OF MYCELIOID FUNGUS TRICHODERMA LONGIBRACHIATUM AS PRODUCER OF CARBOHYDRASES COMPLEX CONTAINING CELLULASE, BETA-GLUCANASE, XYLANASE, PECTINASE AND MANNANASE 2001
  • Okunev O.N.
  • Sinitsyn A.P.
  • Chernoglazov V.M.
  • Bekkarevich A.O.
RU2195490C2
STRAIN OF MYCELIAL MUSHROOM MYCELIOPHTHORA FERGUSII-PRODUCER OF NEUTRAL CELLULASE, BETA-GLUCANASE AND XYLANASE 2008
  • Sinitsyn Arkadij Pantelejmonovich
  • Okunev Oleg Nikolaevich
  • Chernoglazov Vladimir Mikhajlovich
  • Tsurikova Nina Vasil'Evna
  • Novozhilov Evgenij Vsevolodovich
  • Sinitsyna Ol'Ga Arkad'Evna
RU2361915C1
TRICHODERMA REESEI MYCELIAL FUNGUS STRAIN - PRODUCER OF ENDOGLUCANASE, XYLANASE AND PECTINASE COMPLEX FOR PRODUCTION OF PROTEIN ADDITIVES BASED ON CEREAL AND LEGUME RAW MATERIAL FOR USE IN FODDER PRODUCTION 2018
  • Sinitsyn Arkadij Pantelejmonovich
  • Tsurikova Nina Vasilevna
  • Kostyleva Elena Viktorovna
  • Sereda Anna Sergeevna
  • Velikoretskaya Irina Aleksandrovna
  • Veselkina Tatyana Nikolaevna
  • Nefedova Lidiya Ivanovna
RU2696074C1
FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION 2012
  • Okunev Oleg Nikolaevich
  • Bekkarevich Aleksandra Olegovna
  • Matys Veronika Jur'Evna
  • Bubnova Tamara Viktorovna
  • Koshelev Anatolij Vladimirovich
  • Nemashkalov Vitalij Alekseevich
  • Rozhkova Aleksandra Mikhajlovna
  • Sinitsyn Arakadij Pantelejmonovich
RU2532840C2
STRAIN OF MYCELIAL FUNGUS TRICHODERMA LONGIBRACHIATUM AS PRODUCER OF CARBOHYDRASES COMPLEX COMPRISING CELLULASES, β-GLUCANASES, XYLANASES, MANNANASES AND PECTINASES 2004
  • Okunev Oleg Nikolaevich
  • Sinitsyn Arkadij Pantelejmonovich
  • Chernoglazov Vladimir Mikhajlovich
  • Bekkarevich Aleksandra Olegovna
RU2287571C2
GENETIC CONSTRUCT FOR EXPRESSION OF A COMPLEX OF ENZYMES OF ENDOGLUCANASES AND XYLANASES IN THE CELLS OF THE FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR OBTAINING COMPLEX ENZYME PREPARATIONS BASED ON IT, INTENDED FOR FODDER PRODUCTION 2017
  • Sinitsyn Arkadij Pantelejmonovich
  • Zorov Ivan Nikitich
  • Rozhkova Aleksandra Mikhajlovna
  • Sinitsyna Olga Arkadevna
  • Shashkov Igor Aleksandrovich
  • Merzlov Dmitrij Andreevich
  • Matys Veronika Yurevna
  • Satrutdinov Ajdar Damirovich
RU2653429C1
STRAIN OF PENICILLIUM VERRUCULOSUM PRODUCER OF PHYTASE AND ENDO-1,4-β-GLUCANASE II COMPLEX AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED 2022
  • Sinitsyn Arkadii Panteleimonovich
  • Zorov Ivan Nikitich
  • Rozhkova Aleksandra Mikhailovna
  • Korotkova Olga Genrikhovna
  • Denisenko Iurii Andreevich
  • Shashkov Igor Aleksandrovich
  • Satrutdinov Aidar Damirovich
  • Matys Veronika Iurevna
  • Sinitsyna Olga Arkadevna
  • Rubtsova Ekaterina Aleksandrovna
RU2810538C2
PENICILLIUM VERRUCULOSUM MX-73 FUNGUS STRAIN PRODUCER OF MODIFIED XYLANASE E WITH HIGH HEAT STABILITY, ENZYME PREPARATION BASED THEREON FOR USE IN FOOD AND FEED INDUSTRY AND METHOD FOR PRODUCTION THEREOF 2018
  • Sinitsyn Arkadij Pantelejmonovich
  • Osipov Dmitrij Olegovich
  • Korotkova Olga Genrikhovna
  • Zorov Ivan Nikitich
  • Rozhkova Aleksandra Mikhajlovna
  • Sinitsyna Olga Arkadevna
  • Shashkov Igor Aleksandrovich
RU2711578C1
MYCELIUM FUNGI STRAIN TRICHODERMA LONGIBRACHIATUM AS CELLULASE, BETA-GLUCANASE AND XYLANASE PRODUCER 2005
  • Okunev Oleg Nikolaevich
  • Bakkarevich Aleksandra Olegovna
  • Sinitsyn Arkadij Pantelejmonovich
  • Chernoglazov Vladimir Mikhajlovich
RU2303065C1

RU 2 705 262 C2

Authors

Leontevskij Aleksej Arkadevich

Lisov Aleksandr Viktorovich

Belova Olga Valerevna

Kovalevskaya Zhanna Ivanovna

Lisova Zoya Aleksandrovna

Solonin Aleksandr Sergeevich

Zakharova Marina Viktorovna

Shadrin Andrej Mikhajlovich

Dates

2019-11-06Published

2018-03-29Filed