FIELD: biotechnology.
SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, namely to genetic construct for ensuring the expression of targeted homologous and heterologous genes in the cells of the recipient fungus Penicillium verruculosum. Present construct comprises a target coding sequence comprising a serially connected eglII gene encoding a highly active endo-1,4-β-glucanase II, and the xyIE Penicillium canescens gene encoding the uninhibited endo-1,4-β-xylanase E. Invention also relates to recombinant strains of Penicillium verruculosum EX13, VKM F-4765D, and Penicillium verruculosum EX35, VKM F-4766D. These strains are intended for the production of homologous highly active endo-1,4-β-glucanase II and uninhibited endo-1,4-β-xylanase E Penicillium canescens. Present invention allows the preparation of uninhibited endo-1,4-β-xylanase E of Penicillium canescens and highly active endo-1,4-β-glucanase II of Penicillium verruculosum.
EFFECT: ensuring expression of the target homologous and heterologous genes in the cells of the recipient fungus Penicillium verruculosum.
4 cl, 2 dwg, 2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| PENICILLIUM VERRUCULOSUM MX-73 FUNGUS STRAIN PRODUCER OF MODIFIED XYLANASE E WITH HIGH HEAT STABILITY, ENZYME PREPARATION BASED THEREON FOR USE IN FOOD AND FEED INDUSTRY AND METHOD FOR PRODUCTION THEREOF | 2018 |
|
RU2711578C1 |
| STRAIN OF PENICILLIUM VERRUCULOSUM PRODUCER OF PHYTASE AND ENDO-1,4-β-GLUCANASE II COMPLEX AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2022 |
|
RU2810538C2 |
| GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE | 2013 |
|
RU2550044C2 |
| PENICILLIUM VERRUCULOSUM FUNGUS STRAIN AS PRODUCER OF COMPLEX OF PHYTASE A AND ENDO-1,4-β-XYLANASE E AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2023 |
|
RU2819918C1 |
| STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM EE-105, PRODUCER OF A COMPLEX OF HIGHLY EFFECTIVE ENDOGLUCANASES AND AN ENZYME PREPARATION BASED THEREON FOR USE AS A FODDER ADDITIVE IN CEREAL FODDERS | 2018 |
|
RU2696036C1 |
| PENICILLIUM CANESCENS MTCBHI FUNGUS STRAIN PRODUCER OF THERMALLY STABLE CELLOBIOHYDROLASE I AND AN ENZYME PREPARATION BASED THEREON FOR BIOCONVERSION OF RENEWABLE VEGETAL RAW MATERIAL INTO SUGARS | 2019 |
|
RU2741078C1 |
| FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION | 2012 |
|
RU2532840C2 |
| RECOMBINANT STRAIN OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM (VERSIONS) AND A METHOD OF PRODUCING ENZYME PREPARATION WITH ITS USE (VARIANTS) | 2015 |
|
RU2646136C2 |
| STRAIN OF TRICHODERMA LONGIBRACHIATUM TW-14-220 FILAMENTOUS FUNGUS - PRODUCER OF CELLULASES, BETA-GLUCANASES AND XYLANASES FOR FEED PRODUCTION AND A METHOD FOR OBTAINING A FEED COMPLEX ENZYME PREPARATION | 2017 |
|
RU2654564C1 |
| NEW RECOMBINANT STRAIN OF FILAMENTOUS FUNGI PENICILLIUM CANESCENS CS15, PRODUCING CELLULASE CLOSTRIDIUM THERMOCELLUM AND METHOD OF ITS CULTIVATION | 2015 |
|
RU2612158C1 |
