METHOD FOR IMPROVING ANTIBODY STABILITY Russian patent published in 2020 - IPC G01N33/68 G16B15/00 G16C99/00 

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry, particularly to a method of selecting one or more antibodies resistant to degradation. Said method includes steps of: a) taking two or more antibodies, b) each residue Asn and Asp in the Fv domain of the antibody is determined conformational mobility C_α-atom using ensemble of models based on homology, c) each residue of Asn and Asp in the Fv antibody domain is used to determine the size of the amino acid residue adjacent to the Asn or Asp residue from the C-terminal side, d) selecting one or more antibodies in which C_α-atom is conformationally fixed and in which on the C-end side to Asn or Asp adjoins an amino acid residue with a surface area accessible to solvent of 111 Ų or more, where the conformational mobility is the mean-square deviation (RMSD) of the corresponding C_α-atoms of Asn/Asp residues in an ensemble of models based on homology.

EFFECT: invention enables efficient selection of one or more antibodies resistant to degradation.

1 cl, 8 dwg, 3 tbl, 8 ex