CELL PRODUCT FOR LOADING AND ACTIVATION OF HUMAN DENDRITE CELLS Russian patent published in 2020 - IPC C12N5/784 C12N5/09 A61K35/15 A61P35/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine and biotechnology, namely to creation of cell products from cell lines of human skin melanoma lines. Cell product consists of equal parts of lysates of human skin melanoma cell lines deposited in a specialized collection of vertebrate cell cultures of the Russian collection of cell cultures under the corresponding registration numbers: 226 AB mel – under number RKKK(P)725D, 283 mel – under number RKKK(P)726D, Mel 311 – under number RKKK(P)703D, 515 mel – under number RKKK(P)757D, mel 520 DVA – under RKKK(P)779D, 685 mel GSN number – RKKK(P)781D, 686 mel FLA – under number RKKK(P)782D, 860 mel BII – under number RKKK(P)783D, mel 929 SVU – under number RKKK(P)780D. Lysates of said cell lines are prepared by multiple freezing-thawing with determination of cell viability by flow cytometry, one aliquot of the cell product contains the total number of cells 30 million, which are in 500 mcl of normal saline.

EFFECT: cell product enables effective loading and activation of human dendritic cells with cancer-testicular antigens (CTA) expressed in malignant growth cells that can provide creation of universal anti-tumor dendritic-cell human vaccines.

1 cl, 12 dwg, 2 ex