FIELD: medicine.
SUBSTANCE: present invention refers to medicine, namely to a nerve cell staining method. Method of nerve cell coloring involves treatment of sections with dye solution, dehydration and conclusion in balsam, wherein sections with thickness of 12 to 20 mcm are stained from 8 to 14 minutes in a thermostat at temperature of 22±0.5 °C in freshly prepared 0.2–0.5 % amidol solution, after coloring sections are successively rinsed for 30 seconds in two portions of distilled water.
EFFECT: disclosed method is effective for nerve cells staining.
3 cl, 1 dwg, 2 tbl, 1 ex
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Authors
Dates
2020-03-10—Published
2019-10-31—Filed