METHOD FOR MODELING PERITONITIS Russian patent published in 2020 - IPC G09B23/28 A61B17/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to experimental surgery, and can be used for modeling peritonitis. Microbial suspension of E. coli and B. fragilis is introduced into the animal's abdominal cavity. Experimental animals used are male Wistar rats aged 6–9 months who undergo laparotomy. Blind intestine is removed into the wound, and a serous-muscular shell incision up to 1 cm is made in an avascular region of the blinded canopy region. Intestinal wound is closed with a continuous blanket suture. Then E. coli and B. fragilis suspension containing 0.5 ml 10⁹ microbial bodies of each is introduced into the abdominal cavity. Laparotomy wound is closed. Method enables to obtain a model of postoperative diffuse peritonitis with characteristic signs of the clinical course of the disease by introducing into the abdominal cavity a pathogenic microflora consisting of hospital E. coli and B. fragilis strains, which leads to development of peritoneal symptoms with rapidly increasing intoxication, intestinal motility disorder, expressed microcirculatory disorders with metabolic acidosis.

EFFECT: method is proposed for simulating peritonitis.

1 cl, 5 dwg, 1 ex