METHOD FOR DETERMINING GASTROINTESTINAL DYSBIOSIS Russian patent published in 2020 - IPC A61B5/00 

FIELD: medicine.

SUBSTANCE: disclosed is a method for determining the probability of dysbiosis of the gastrointestinal (GI) tract in a subject, a method for quantitative determination of dysbiosis, a method for obtaining information significant for diagnosis, monitoring and/or characterization of diseases and conditions associated with gastrointestinal microbiota disorders, diagnostic, monitoring and/or characteristics of diseases and conditions, associated with microbiota disorders of the GI tract, as well as a system for determining the probability of dysbiosis of the GI tract in a subject. Providing a set of experimental data containing at least one microbiota profile. Said microbiota profile is a profile of relative levels of a plurality of microorganisms or groups of microorganisms in a sample from the said individual's gastrointestinal tract. Application of said set of experimental data of at least one load vector, which is at least one orthogonal latent variable, determined by regression analysis of the levels of said plurality of microorganisms or groups of microorganisms in corresponding samples from the LC tract of a plurality of normal subjects. This provides a first set of projection data. Providing said first plurality of projection data. Calculating the Q-residue of the microbiota profile from said first plurality of projection data. Comparison of said Q-residue of microbiota profile with threshold value of Q-residue at transition from normobiotic to dysbiotic state, determined in corresponding analysis of said plurality of microorganisms or groups of microorganisms in corresponding samples from the GI tract of multiple normal subjects and/or subjects suffering from dysbiosis. Hotelling's T² calculation from said first plurality of projection data for microbiota profile from dispersion, explained by said orthogonal hidden variables determined by regression analysis of levels of said plurality of microorganisms or groups of microorganisms in corresponding samples from the LC tract of a plurality of normal subjects. Comparison Hotelling's T² for the microbiota profile with the Hotelling's T² threshold value at transition from the normobiotic to the dysbiotic state, determined in corresponding analysis of said plurality of microorganisms or groups of microorganisms in corresponding samples from the LC tract of a plurality of normal subjects and/or a plurality of subjects suffering from dysbiosis. Moreover profile microbiota, Q-residue or Hotelling's T² which is greater than said respective threshold values indicating the probability of dysbiosis.

EFFECT: group of inventions makes it possible to accurately and effectively determine the probability of gastrointestinal dysbacteriosis and to quantify its degree.

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